Biotechnology of Green Fluorescent Protein (GFP): from glowing animals to multicolor probes for signaling, protein-protein interaction and drug screening (4 h)
Discovery of Green Fluorescent Protein (GFP) has revolutionized Biomedical Sciences, making it possible optical visualization and imaging of organs, cells, sub-cellular structures and proteins in living animals and plants. Generation of full color palette of fluorescent proteins (PFs) and biotechnological engineering of fluorescent probes, based on FRET (fluorescence resonance energy transfer) and cpGFP (circularly permuted GFP), enabled monitoring of dynamic changes of intracellular concentrations of ions and second messengers. Split-GFP probes allowed dynamic visualization of interaction between proteins and organelles. All this made the fluorescent proteins an integral part of the discovery process from basic research to biotechnological tools and products. This course will provide a basic knowledge on structure and usage of GFP and its analogues/derivatives. Examples will include multicolor transgenic animals, structure and usage of probes for investigation of cellular signaling processes, protein-protein interaction and drug screening
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